eLC-002

PRINCIPLE AND OPERATION OF FRC-10A/40 FRACTION COLLECTOR WITH LC-20AP/AR PREPARATIVE LC SYSTEM

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  • Author: Shimadzu CAS
  • Level: Beginner
  • Study time: 5 hours
  • Video time: 2 hours
  • Exams: Yes
  • Certification: Yes
Course overview
This course offers practical knowledge to familiarize users with operation of Shimadzu LC-20AP/AR Prep LC with FRC-10A/40.
Learning Outcome
At the end of the course, you will be able to:
  • Understand the Principle & Instrumentation of Preparative Liquid Chromatography (Prep LC) with FRC-10A.
  • Familiarize with Shimadzu Prep LC Systems & Configurations for efficient method development and operation.
  • Operate and apply Prep LC with FRC-10A, ensuring proper system startup, shutdown, and data acquisition.
  • Understand the Instrumentation of Prep HPLC with FRC-40, including system components and workflow.
  • Perform LC Purification, covering fundamentals, scaling strategies, and optimization for various purification needs.
  • Apply Semi-Micro & Analytical Scale Techniques in LC purification, enhancing separation efficiency.
  • Master Semi-Preparative Scale Chromatography for effective compound isolation and purification.
  • Troubleshoot common issues in Prep LC analysis and optimize system performance.

Materials Required for eLC-002
e-Learning Course:

Instrumentation

  • An integrated or modular liquid chromatograph equipped with UV-Vis or photodiode array (PDA) detector
  • FRC-10A/40 Fraction Collector With LC-20AP/AR Preparative LC System
  • Shim-pack C18 column with 50 mm length, 3.0 mm ID, 2.2 µm (P/N 228-41606-92) or equivalent

Operation Software

  • LabSolutions LC (with License)

Chemical & Reagent

  • Methanol solvent
  • Caffeine and parabens standard
  • 1.5 ml sample vial with cap (P/N 226-54110-31)
Notes
  • We provide demo data files for qualitative and quantitative data processing (data processing is done in LabSolutions LC software)
  • If LabSolutions LC software is not available, course contents are still beneficial to all HPLC users

Course contents

Instructors for this course

Meet Your Instructors

All of our instructors are highly qualified professionals, widely acknowledged in their field.
Dr Desmond Sim
Dr. Zhan

Request for Live Training

For our customers who require on-site or online training, we provide a live option.
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