PRINCIPLE AND OPERATION OF FRC-10A/40 FRACTION COLLECTOR WITH LC-20AP/AR PREPARATIVE LC SYSTEM
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Author: Shimadzu CAS
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Level: Beginner
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Study time: 5 hours
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Video time: 2 hours
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Exams: Yes
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Certification: Yes
Course overview
This course offers practical knowledge to familiarize users with operation of Shimadzu LC-20AP/AR Prep LC with FRC-10A/40.
Learning Outcome
At the end of the course, you will be able to:
- Understand the Principle & Instrumentation of Preparative Liquid Chromatography (Prep LC) with FRC-10A.
- Familiarize with Shimadzu Prep LC Systems & Configurations for efficient method development and operation.
- Operate and apply Prep LC with FRC-10A, ensuring proper system startup, shutdown, and data acquisition.
- Understand the Instrumentation of Prep HPLC with FRC-40, including system components and workflow.
- Perform LC Purification, covering fundamentals, scaling strategies, and optimization for various purification needs.
- Apply Semi-Micro & Analytical Scale Techniques in LC purification, enhancing separation efficiency.
- Master Semi-Preparative Scale Chromatography for effective compound isolation and purification.
- Troubleshoot common issues in Prep LC analysis and optimize system performance.
Materials Required for eLC-002
e-Learning Course:

Instrumentation
- An integrated or modular liquid chromatograph equipped with UV-Vis or photodiode array (PDA) detector
- FRC-10A/40 Fraction Collector With LC-20AP/AR Preparative LC System
- Shim-pack C18 column with 50 mm length, 3.0 mm ID, 2.2 µm (P/N 228-41606-92) or equivalent
Operation Software
- LabSolutions LC (with License)
Chemical & Reagent
- Methanol solvent
- Caffeine and parabens standard
- 1.5 ml sample vial with cap (P/N 226-54110-31)
Course contents
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